murine Adenosine Deaminase (mADA)

	19F Position and Residue Number	Chemical Shift Folded	Chemical Shift Unfolded
Apo	6-F-W117	-45.15	-46.15
Apo	6-F-W161	-48.19	-46.27
Apo	6-F-W264	-47.59	-46.27
Apo	6-F-W272	-47.03	-46.45
DCF	6-F-W117	-44.82
DCF	6-F-W161	-47.73
DCF	6-F-W264	-47.55
DCF	6-F-W272	-46.85
PR	6-F-W117	-45.60
PR	6-F-W161	-47.98
PR	6-F-W264	-47.55
PR	6-F-W272	-46.85

Sample conditions: 20 mM Tris-HCl, pH 7.4, 2 mM DTT, 5% D2O, 20 degC.
Referenced to internal 0.2 mM 4-19F-Phe.
Biochemistry (2004) 43, 1432-1439
J. Mol. Biol. (2005) 345, 599-610

Dihidrofolate Reductase (DHFR)

	19F Position and Residue Number	Chemical Shift Folded	Chemical Shift Unfolded
Apo	6-F-W22	-45.6	-46.1
Apo	6-F-W30	-44.8	-46.3
Apo	6-F-W47	-49.2	-46.3
Apo	6-F-W74	-45.0	-46.4
Apo	6-F-W133	-46.9	-46.5
MTX	6-F-W22	-46.7
MTX	6-F-W30	-44.6
MTX	6-F-W47	-49.5
MTX	6-F-W74	-44.8
MTX	6-F-W133	-47.5
NADPH	6-F-W22	-44.0
NADPH	6-F-W30	-45.0
NADPH	6-F-W47	-49.3
NADPH	6-F-W74	-43.1
NADPH	6-F-W133	-46.9
MTX/NADPH	6-F-W22	-44.2
MTX/NADPH	6-F-W30	-45.0
MTX/NADPH	6-F-W47	-49.6
MTX/NADPH	6-F-W74	-42.9
MTX/NADPH	6-F-W133	-47.2
DHF	6-F-W22	-46.2
DHF	6-F-W30	-44.8
DHF	6-F-W47	-49.6
DHF	6-F-W74	-45.1
DHF	6-F-W133	-47.1
NADP	6-F-W22	-45.1
NADP	6-F-W30	-46.5
NADP	6-F-W47	-49.6
NADP	6-F-W74	-43.0
NADP	6-F-W133	-47.1

Sample conditions: 50 mM phosphate pH 7.2, 0.1 M KCl, 0.1 mM EDTA, 1 nM DTT, 10%D2O
Referenced to external TFA at 0 ppm.

Intestinal Fatty-acid Bindng Protein (I-FABP)

	19F Position and Residue Number	Chemical Shift Folded	Chemical Shift Unfolded
Apo	4-F-F2	-37.52
Apo	4-F-F17	-37.29
Apo	4-F-F47	-38.84
Apo	4-F-F55	-40.72
Apo	4-F-F62	-47.16
Apo	4-F-F68	-44.80
Apo	4-F-F93	-38.26
Apo	4-F-F128	-41.38
Oleate	4-F-F2	-37.75
Oleate	4-F-F17	-35.65
Oleate	4-F-F47	-36.76
Oleate	4-F-F55	-39.24
Oleate	4-F-F62	-45.65
Oleate	4-F-F68	-40.16
Oleate	4-F-F93	-38.04
Oleate	4-F-F128	-39.43

Sample conditions: 20 mM potassium phosphate pH 7.3, 0.25 mM EDTA, 8%D2O, 20degC.
Referenced to internal 6-19F-Tryptophan at -46.293 relative to TFA at 0 ppm.
Biochemistry (2005) 44, 2369-2377

PapD

	19F Position and Residue Number	Chemical Shift Folded	Chemical Shift Unfolded
Apo	6-F-W36	-46.7	-46.37
Apo	6-F-W128	-43.66	-46.33

Sample conditions: 30 mM MOPS-HCl pH 7.0, 10%D2O, 20 degC.
Referenced to internal 0.3 mM 4-19F-Phe.
Proc. Natl. Acad. Sci. USA (2002) 99, 709-714